Regulation of callose synthases and β-1,3-glucanases during aphid infestation on barley cv. Clipper
نویسندگان
چکیده
Plant material and aphid species 8 RNA extraction and measurements of RNA concentration with Nano-Drop 9 RT-PCR and agarose gel electrophoresis 10 Real-Time PCR 11 Bioinformatics: searching for sequences, sequence alignment 13 Results 15 Expression of callose synthase 15 Callose synthase expression analysis with RT-PCR 17 Callose synthase expression analysis with Real-Time PCR 20 Expression of β-1,3-glucanase 23 β-1,3-glucanase expression analysis with RT-PCR 25 β-1,3-glucanase expression analysis with Real-Time PCR 26 Summary of the results 30 Rhopalosiphum padi virus (RhPV) detection 31
منابع مشابه
Stronger induction of callose deposition in barley by Russian wheat aphid than bird cherry-oat aphid is not associated with differences in callose synthase or beta-1,3-glucanase transcript abundance.
The effects of infestation by the bird cherry-oat aphid (BCA), (Rhopalosiphum padi L) and the Russian wheat aphid (RWA) (Diuraphis noxia Mordvilko) on callose deposition and transcription of genes related to callose accumulation were investigated in barley (Hordeum vulgare L. cv. Clipper). The BCA, which gives no visible symptoms, induced very limited callose deposition, even after 14 days of i...
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